Parainfluenza virus (type I, II, III) nucleic acid detection kit (PCR-fluorescent probe method)
【Clinical manifestations】
Parainfluenza viruses can mainly cause lower respiratory tract infections in children, and its pathogenicity is second only to respiratory syncytial virus (RSV) . Human parainfluenza virus can cause upper respiratory tract infections such as recurrent colds and sore throats. It can also cause severe recurrent infections of pneumonia, bronchitis, and bronchiolitis of lower respiratory tract diseases. The lower respiratory tract infections caused mainly occur in the elderly and in people with immunodeficiency.
【Features】
This kit uses real-time fluorescent PCR technology, with highly conserved regions of the parainfluenza virus ( types I , II , and III ) as target regions, designing specific primers and fluorescent probes, and then performing PCR amplification on the target sequence , while using Taqman Fluorescent probe technology monitors the accumulation of amplified products in real time to achieve qualitative detection of respiratory syncytial virus nucleic acids in samples . The reaction system of this kit contains dU-UNG enzyme anti-pollution system to avoid false positive results.
【Product Performance】
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Sensitivity
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1.0x10 3 copies / mL
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Linear range
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1.0x10 3 copies / mL ~ 1.0x 10 9 copies / mL
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Accuracy
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The detection result coincidence rate is 100%
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Precision
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Coefficient of variation within and between batches CV≤5%
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Specificity
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100% specificity , no cross reaction with influenza A virus, influenza B virus, adenovirus , human Boca virus, Epstein-Barr virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, etc.
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Anti-interference
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Oxymetazoline hydrochloride spray, budesonide nasal spray , zanamivir , ribavirin did not interfere with the test results
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【Product information】
Test specimen: throat swab
Technical principle: PCR- fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480 ; Roche LightCycler 480 ; ABI 7500 ; Shanghai Hongshi SLAN-96S ; Bioer LineGene 9600, etc.
