Respiratory syncytial virus nucleic acid detection kit (PCR-fluorescent probe me
Respiratory syncytial virus nucleic acid detection kit (PCR-fluorescent probe method)

Respiratory syncytial virus nucleic acid detection kit (PCR-fluorescent probe method)

【Clinical manifestations】
  Respiratory syncytial virus (Respiratory syncytial virus, RSV) is a common pathogen that causes respiratory tract infections. It is also the main pathogen of respiratory infections in infants and young children. RSV can cause respiratory infections at any age from newborns to adults. It can cause upper respiratory symptoms such as fever, nasal congestion, pharyngitis and otitis media, as well as lower respiratory symptoms such as asthma, bronchitis, bronchitis and pneumonia. Respiratory syncytial virus infection targets mainly infants and young children. It is the main pathogen of lower respiratory tract infection and viral pneumonia in infants and young children. It can cause interstitial pneumonia and bronchiolitis, and the reinfection rate is very high. Acute infection in adults is also very common. After infection, it mainly manifests as upper respiratory tract infection. The elderly can cause severe pneumonia and develop into adult respiratory distress syndrome (ARDS). Respiratory syncytial virus can cause epidemic asthmatic pneumonia, resulting in the death of infants and young children, especially newborns. In addition, respiratory syncytial virus can also cause bronchial asthma.
【Features】
  This kit utilizes real-time fluorescence PCR technology to respiratory syncytial virus genomic region is highly conserved target region, the design of specific primers and fluorescent probes, and target sequences for PCR amplification, through Taqman fluorescent probe to monitor real-time expansion techniques Accumulation of augmentation products enables qualitative detection of respiratory syncytial virus nucleic acid in the sample. The reaction system of this kit contains dU-UNG enzyme anti-pollution system to avoid false positive results.
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result coincidence rate is 100%

Precision

Coefficient of variation within and between batches CV≤5%

Specificity

100% specificity , no cross reaction with influenza A virus, 

influenza B virus, adenovirus , human Boca virus, Epstein-Barr virus, 

Mycoplasma pneumoniae, Chlamydia pneumoniae, etc.

Anti-interference

Oxymetazoline hydrochloride spray, budesonide nasal spray , 

zanamivir , ribavirin did not interfere with the test results

【Product information】

Test specimen: throat swab

Technical principle: PCR- fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480 ; Roche LightCycler 480 ; ABI 7500 ; Shanghai Hongshi SLAN-96S ; Bioer LineGene 9600, etc.

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